The Pressure Pulsation Myograph System - 112PP is an ideal system used to study the structure and function of isolated sections of small vessels (diameter >40 μm) under near-physiological conditions. Vessel diameters can be measured in response to pharmacological and physiological stimuli.

In addition the system allow simulation of pulsations between 50 and 600 BPM with a pressure difference up to 60 mmHg.

A built-in heating system maintains the chamber temperature, eliminating the need for continuous and often costly superfusion. The chamber cover includes ports for superfusion, for rapid draining and filling, for cumulative addition of drugs and for oxygenation. To facilitate cleaning, the chamber is made of acid resistant stainless steel.

Measurements are continuously recorded by a computer with dimension analysis software - MyoVIEW.

As an option, the FlowMeter - 162FM can be added to the system to measure flows between 15 - 4000 μl/min. - flow cannot be measured during pulzation though.

Because of the nature of the technique, physiological responses such as the myogenic response and flow-mediated dilation can be examined. The state-of-the-art Pressure Myograph software MyoVIEW will collect data such as vessel wall thickness, changes in vessel and lumen diameter, intravascular pressure, and a host of other calculated parameters, such as shear stress and vascular resistance, setting pressure or perfusion myography apart from standard organ bath techniques.

The Myograph & Pressure package

Myograph chamber, Pressure Interface, chamber cover, calibration kit, internal and external tubing, set of polished cannulae.

The Acquisition & Analysis package

Upright Zeiss, Nikon microscope or Motic (contact DMT for further specifications) with USB camera, PC and MyoVIEW

• Shear stress, luminal pressure, mechanical stretch and tension in longitudinal direction…. all in one system

• Ergonomic design to facilitate easy and quick mounting of arteries

• Low volume of chamber fluid to minimize amount of reagents

• Pulsatile flow studies with pulse rates between 50 and 600 per minute

"Non-endothelial endothelin counteracts hypoxicvasodilation in porcine large coronary arteries" Hedegaard ER, Stankevicius E, Simonsen U, Fröbert O.