The Promethion Core Mouse Metabolic System gives you the truest and most trustworthy results from your mouse phenotyping studies. At the heart of the system is the highest resolution metabolic measurement instrumentation, yielding accurate resting and active energy expenditure values.

Second-by-second behavioral measurements provide the highest data granularity. Promethion Core tightly synchronizes all metabolic and behavioral data, allowing you to pinpoint and correlate metabolism changes with behavioral events. The Environmental Sensor Array (ESA) data is also available in real-time to correlate any changes in the environment with the metabolic and behavioral changes. All the raw data is saved for further data mining.

Other systems try to match Promethion Core’s performance by employing an oversampling technique, for example, continuous gas measurements of each cage. But due to these systems’ low-resolution gas analyzers and requisite slow air-exchange rates, continuous oversampling simply results in useless and redundant data (see video “The Effects of Cage Air-Flow Rate”).

The system is expandable to enable microbiome research with our biocontainment cage plus methane analyzer, fuel utilization research with our stable isotope gas analyzer, and temperature/light-controlled studies with our Environmental Control Cabinet. You can even connect 3rd party equipment such as telemetry and optogenetic systems.

The entire system is compact and self-contained to save valuable lab space, allowing up to 16 cages in an environmental cabinet. This provides 33% more housed cages than competitor systems, maximizing your experimental power. Your workflow is efficient with swappable components and magnetic mounts for quick exchange of hoppers and measurement devices.

The new Promethion Live software enables you to control all aspects of your study. The simplified interface maximizes experiment success with intuitive system and experiment setup. One-step gas calibration ensures accurate respirometry measurements. Status indicators for vital components verify system performance. Constant monitoring of all parameters means you will always know the well-being of each animal. Routine experiment configurations can be easily saved and reused.

With Promethion Live, you can explore comparative trends during an experiment looking at every parameter measured, such as running speed, food consumed, or energy expended (data shown). You’re empowered with the real-time experiment and animal information needed to make key decisions at any time, from any place.

Key Components

• Promethion Core instrumentation includes:

• Triple gas single path analyzer

• 8-channel flow generator and multiplexer

• Promethion Live onboard computer and memory

• Standard size home cage

• Precision mass monitors

• Magnetic mount food hopper

• Magnetic mount water bottle with sipper tube

• 2- or 3-dimensional infrared beam break array

• Promethion Live software platform

• Desktop computer with monitor

Optional Components

• Body mass habitat

• Running wheel

• Access control door for food hopper or water bottle

• Environmental Sensor Array (ESA)

• Environmental control cabinet

• Telemetry bases for body temperature and heart rate

• Stable Isotope Analyzer (13CO2)

• Methane Analyzer

• Optogenetic compatible cage lid

SableHD™ High Definition Technology

SableHD™ High Definition Technology is built into each Promethion system, allowing you to capture data at 0.001 to 0.0001 resolution or better – setting the standard that no other analyzers can match.  You’ll see every aspect of metabolism and / or behavior more clearly, with finer detail, quality and trustworthiness.  All of your critical measurements and analyses are captured and recorded more frequently and to a higher level.  Combined with Sable’s unexcelled accuracy and precision, you’ll have complete confidence in your data. You’ll avoid the pitfalls associated with low-resolution analyzers, such as massive data averaging to compensate for poor signal stability, data inconsistencies from one study to the next, data drift, and poor reliability of data. Using SableHD™ technology, your study gets done right the first time, without the fear of having to rerun your entire study.

Raw Data Storage

Raw Data Storage means there are no secret algorithms, or hidden pre-conditioning of data, just data that is transparent and fully traceable. The parameters important for your research can be extracted any way you like – energy expenditure, metabolic substrate selection, food and water uptake, meal and drinking patterns, position, total activity and wheel-running, live body mass – even fully automated behavioral analysis is possible.  All information is perfectly synchronized to the system’s heartbeat of one second. Nothing is ever lost again. If you decide to reanalyze the collected data or verify/validate for QC purposes, the original data is fully accessible in its original highly resolved state.

Water Vapor Dilution Compensation

Water Vapor Dilution Compensation allows you to avoid sample gas drying and significantly increase accuracy of results.  This is made possible by the sensitive, stable water vapor pressure analyzer built into each Promethion gas analyzer. The system uses the readings from the water vapor pressure analyzer, together with data from the analyzer’s barometric pressure sensor, to perform water vapor dilution compensation for O2 and CO2 concentrations, plus flow rate correction for the presence of water vapor.

Pull-Mode Flow Generators 

Pull-Mode Flow Generators(patent pending) for mouse or rat cages allow the use of essentially any standard live-in cage bottom for measurement.  All you have to do is to replace the lid. No handling of the mouse, no unfamiliar new environment.  This is a significant advancement over conventional systems, which use sealed metabolic cages – causing stress effects and requiring long acclimation times.

Fully Configured Systems 

Fully Configured Systems are designed for a broad range of applications, including metabolic phenotyping, behavioral analysis, calorimetry, respirometry, or gas analysis.  Multiplexed or continuous, for mice, rats or humans, we offer a range of Promethion systems to accomplish your research goals.

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